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The oocyte maturation process – From GV to the MII stage.

The oocyte maturation process – From GV to the MII stage.
Submitted By: Thorir Hardarson, Phd.
Website: Fertility Center Scandinavia
Date: 28th August 2010
Time: 1:36

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ABOUT THIS PODCAST

Before an oocyte can be fertilised it has to go through both the cytoplasmic maturation and the nuclear maturation, which this sequence shows. Initially the germinal vesicle nucleus breaks down and disappears, which symbolizes the start of the nuclear maturation. The oocyte has already increased in volume approximately 20 times and reached a diameter of approximately 0,1 mm. This increase in volume takes place when the cytoplasmic maturation process starts several days prior. From a morphological standpoint the end of the first cellular reduction is seen by the appearance of a large cell, the oocyte itself and a tiny attached cellular structure, the so called polar body. The oocyte has reached the MII stage and is ready for fertilization.

Finally we see at high magnification the relatively fast extrusion of the polar body. Despite it´s small size, the polar body it normally contains half of the chromosomes of the oocyte.

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